Pnp Specn Lab

Spectroscopy and PNP

CHE315
Section 03
Professor
Submitted By:
Submitted to:
October 21, 2011

RESULTS
Part I. mensuration the Absorbance Spectra of the acid and sanctioned Forms of para-Nitrophenol (PNP)
Part A: tyrannical Spectra for Acidic (phenol) and Basic (phenolate) PNP
An absolute spectra was generated for the acidic and rudimentary form of PNP. reveal Fig. 1 and Fig. 2.
See skirt 1 for the absorbance data results and Table 2 for the absorbance mean values of the data results. See the addendum for detailed calculations.
The data results for the acidic PNP absolute spectra shows a ? scoopful of 360 nm at an absorbance of 0.315 and the data results for the basic PNP absolute spectra shows a ? max of four hundred nm at an absorbance of 0.515.

PNP + pH10| | PNP + pH5|
?| Abs.| | ?| Abs.|
340| 0,1183| | 340| 0.248|
350| 0.178| | 350| 0.034|
360| 0.237| | 360| 0.135|
370| 0.317| | 370| 0.086|
380| 0.412| | 380| 0.065|
390| 0.486| | 390| 0.039|
four hundred| 0.515| | 400| 0.034|
410| 0.49| | 410| 0.0135|
420| 0.0412| | 420| 0.033|
430| 0.291| | 430| 0.024|
440| 0.019| | 440| 0.0215|
450| 0.101| | 450| 0.025|
460| 0.04| | 460| 0.024|
470| 0.07| | 470| 0.022|
480| 0| | 480| 0.018|
490| 0.



01| | 490| 0.017|
500| 0.012| | 500| 0.024|
Table 1: commanding Spectra Data Results of acidic and basic PNP|
| Readings| PNP + pH10 | Readings| PNP + pH10 |
1| 0.081| 1| 0.081|
2| 0.084| 2| 0.084|
3| 0.083| 3| 0.083|
Table 2: Absolute Spectra Means Data Results of acidic and basic PNP |

| ?max = 400 nm
Abs. = 0.515

|
Figure 1: Absolute Spectra for Acidic PNP | Figure 2: Absolute Spectra for Basic PNP|

A plot of the acidic spectra and the basic spectra on the same plot determined the pI to be 345 nm. See Fig. 3.

Figure 3: Absolute Spectra for Acidic and Basic PNP

Part B: Basic - Acidic Difference...If you want to occupy a full essay, order it on our website: Ordercustompaper.com



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