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Dax soni
Chem. 261
Purpose: - The purpose of this experiment is to extract and mea veritable the caffein in a tea theme by separate caffeine from the tea bag and to know the amount of caffeine present in a tea bag by using extraction technique, centrifugation techniques, and by purifying caffeine by sublimation.
Reference: - Williamson, k. Minard R. Masters K. Macro sale and micro sale. innate experiment 2007, 5th edition pg 135-171.
Procedure: -
First, after body of water with boiling worry and Na2co3 boils, remove beaker from anchor bath, remove stick and then add tea bag.
Remove tea bag and squeeze into beaker. Hirsh funnel can be used to certification tea bag while pressing down on it.
later removing the first tea bag, return the same ascendant to the sand bath with boiling stick, reheat to boiling, remove from sand bath, remove boiling stick and add a endorse bag.
Do not use more than 1 or 2 ml of very hot water to scour tea bag.
Total volume of dark tea root word should be no more than 12 ml.
Pour tea solution into two kit 15 ml extractor tube splitting the volume roughly in fractional in each centrifuge tube and cool on ice.
Add 2 ml of CH2CL2 to each tube and hack and shake vigorously or rapidly vortex tubes uncrowned tubes for 15 seconds each so both layers mix well.




centrifugate to break emulsions and obtain two distinct layers.
Cool centrifuge tubes on ice to room temp.
Do not pick out centrifuge tubes more than 4/5 full.
Be sure to balance tubes when using centrifuge
Keep track of your tubes.
After the extraction step, however do not throw any extraction layers out until you have finished the experiment.
Remove the hoy colored bottom layer with glass pipette by first squeezing pipette bulb gently to capture small air bubbles as you direct the glass pennant to the bottom of the tube. Then slowly release pressure in bulb to such...If you want to get a full essay, cabaret it on our website: Ordercustompaper.com



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