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molecules
ISSN 1420-3049 www.mdpi.com/journal/molecules Article
In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymesâ€"Glutathione Transferases (GSTs)
Juzaili Azizi 1, Sabariah Ismail 1,*, Mohd Nizam Mordi 1, Surash Ramanathan 1, Mohd Ikram Mohd express 2 and Sharif Mahsufi Mansor 1
1 2
Centre for Drug Research, Universiti Sains Malaysia, 11800 USM Penang, Malaysia School of chemic Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
* Author to whom correspondence should be addressed; email: sabaris@usm.my; Tel.: +60 46533269; Fax: +60 46568669. Received: 30 November 2009; in revised form: 2 January 2010 / Accepted: 7 January 2010 / produce: 20 January 2010
Abstract: In the present study, we investigate the cause of three different Mitragyna speciosa picks, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase- limited activity in male Sprague Dawley rat liver cytosol in vitro and in vivo.
In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 µg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given...If you want to get a enough essay, order it on our website: Ordercustompaper.com
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